Molecular cloning of the ViaB region of Salmonella typhi |
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Authors: | Yasuhiro Hashimoto Takayuki Ezaki Na Li Hiroyuki Yamamoto |
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Affiliation: | Department of Microbiology, Gifu University School of Medicine, Japan. |
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Abstract: | The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14-kb S. typhi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB-deleted S. typhi GIFU10007-3. Tn5 insertion analysis showed that the 14-kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi polysaccharide. Region 3 is involved in translocation of the Vi polysaccharide to the cell surface. Southern blot hybridization showed that regions 2 and 3 but not region 1, were considerably homologous to the DNA of Vi-positive Citrobacter freundii. |
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Keywords: | Chlamydia trachomatis Wheat germ agglutinin |
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