Keratinization of rat vaginal epithelium IV. Modulation of transglutaminase activity by oestradiol |
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Authors: | S. Vijayasaradhi P. D. Gupta |
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Affiliation: | (1) Centre for Cellular and Molecular Biology, 500 007 Hyderabad, India;(2) Present address: Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021 New York, USA |
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Abstract: | Calcium-dependent transglutaminase activity was found to be present in vaginal homogenates from adult cycling rats. Treatment of immature or adult ovariectomized rats with oestradiol (0.1 μg/g body weight) resulted in 1.5–2-fold enhancement in the enzyme activity. Progesterone treatment (0.1 μg/g body weight) decreased the enzyme activity. Analysis of amino acids produced by proteolytic enzyme digestion of insoluble keratins from rat vaginal tissue indicated the presence of γ-glutamyl-ε-⥿sine dipeptide (4 μmol/g protein) in this protein. These results suggest that oestradiol acts on vaginal tissue and induces the activity of transglutaminase. This enzyme catalyses the formation of γ-glutamyl-ε-lysine crosslinks between keratin polypeptides and thus leads to kerartization of the tissue. |
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Keywords: | Oestradiol keratinization transglutaminase |
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