Separation and characterization of pyrimidine deoxyribonucleoside 2'-hydroxylase and thymine 7-hydroxylase from Aspergillus nidulans |
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| Authors: | P M Shaffer J R Cairns D C Dorman A M Lott |
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| Affiliation: | 1. Centro Integrato di Ricerca, Campus Bio-Medico University, Roma, Italy;2. Istituto per I Sistemi Biologici, Consiglio Nazionale delle Ricerche, Monterotondo, Italy;3. Teaching Committee of Ph.D. School in Natural Science and Engineering, University of Verona, Verona, Italy |
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| Abstract: | Partially purified preparations from Aspergillus nidulans were shown to catalyze two alpha-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2'-hydroxylase (EC 1.14.11.3) and the thymine 7-hydroxylase (EC 1.14.11.6) reactions. These reactions showed an absolute requirement for alpha-ketoglutarate and molecular oxygen and were stimulated by Fe(II), ascorbate and catalase. Both reactions demonstrated a stoichiometry such that for each mole of substrate (deoxyribonucleoside or pyrimidine) hydroxylated one mole of CO2 was produced from alpha-ketoglutarate. These two activities were separated using DEAE-Sephacel chromatography. |
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