Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system |
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Authors: | Stefan-van Staden Raluca-Ioana Bokretsion Rahel Girmai van Staden Jacobus F Aboul-Enein Hassan Y |
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Institution: | Faculty of Chemistry, University of Bucharest, Bucharest, Romania. iustinavanstaden@yahoo.com |
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Abstract: | Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10). |
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