The in vivo phosphorylation sites of bovine alpha B-crystallin |
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| Authors: | C E Voorter W A de Haard-Hoekman E S Roersma H E Meyer H Bloemendal W W de Jong |
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| Institution: | Department of Biochemistry, University of Nijmegen, The Netherlands. |
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| Abstract: | Phosphate content determinations established that in alpha B-crystallin two phosphate groups can be present in vivo in bovine lenses. Comparison of tryptic digests of phosphorylated and unphosphorylated alpha B chains, revealed the location of the two phosphorylation sites in tryptic peptides T2 and T3. Thermolytic digestion and gas-phase sequencing demonstrated that Ser-19 and Ser-45 are the in vivo phosphorylation sites of bovine alpha B-crystallin. This pattern of phosphorylation differs from the previously reported in vitro obtained results. |
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