Analysis of point mutations of the BRCA1 gene by hybridization with hydrogel microarrays |
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| Authors: | O. E. Fedorova O. N. Sinicka L. P. Tihomirova Ya. V. Visnevskaya A. S. Zasedatelev T. V. Nasedkina |
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| Affiliation: | (1) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia;(2) Biomedical Center, University of Latvia, Riga, LV-1067, Latvia;(3) Institute of Clinical Oncology, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478, Russia |
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| Abstract: | BRCA1 mutations are associated with a higher risk of breast (BC) and ovarian cancer in women. Testing for such mutations allows BC prognosis, selection of an individual treatment strategy, and prevention of disease recurrence. Hybridization on a hydrogel microarray was developed for identifying point mutations in BRCA1. The microarray was designed to detect five-point mutations: 185delAG, 300T→G, 4153delA, 4158A→G, and 5382insC. The microarray was tested with 36 control specimens with known genotypes and used to examine 65 BC patients. The results demonstrated the advantage of employing the microarray in analyzing BRCA1 mutations. |
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| Keywords: | breast cancer point mutations multiplex PCR BRCA1 oligonucleotide microarray diagnosis |
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