Localization and dynamics of lipase accumulation in Penicillium solitum hyphae

Lipase localization in the mycelium of Penicillium solitum was studied with cytochemical and immunofluorescent methods. The use of these methods provided detection of the enzyme on the cell walls of the fungus at all periods of its cultivation. The enzyme was not detected in the cell cytoplasm. Measuring of lipase concentrations on the cell walls in relation to the cultivation period suggested that excretion of the exoenzyme into the medium followed its accumulation on the cell walls of the hyphae.