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Cloning of both ends and the thermo-inducible genes A and B of bacteriophage Mu on a multicopy plasmid |
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| Authors: | E van Leerdam T Goosen R H Plasterk P van de Putte |
| Institution: | Department of Molecular Genetics, University of Leiden, Wassenaarseweg 64, 2333 AL Leiden The Netherlands |
| Abstract: | Employing the recombinant DNA technique, two hybrid plasmids were constructed carrying both ends of bacteriophage Mu DNA in two different orientations. The expression of the early Mu genes located on these plasmids is thermo-inducible. |
| Keywords: | Recombinant DNA p AC YC184 and pBR322 plasmid vectors am amber mutation Ap ampicillin bp base pair(s) (M)d (mega)dalton SDS sodium dodecylsulfate Tc tetracycline |
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