中国沙皮犬微卫星DNA分析的遗传多样性

运用20对微卫星引物对来自中国广东省的92份沙皮犬血液样品(骨嘴型沙皮犬21只,骨肉嘴型沙皮犬29只,肉嘴型沙皮犬42只)进行了遗传背景的检测。结果表明,20个多态性微卫星座位共检测到272个等位基因,平均每个座位为13.6个。中国沙皮犬群体的平均杂合度为0.8259,所有微卫星标记座位的多态信息含量(PIC)在0.5352到0.9226之间,说明中国沙皮犬群体存在较为丰富的遗传多样性。遗传距离和聚类分析结果显示,3种类型沙皮犬之间已产生了一定的遗传分化。上述结果为中国沙皮犬细分为不同的品系以及澄清中国沙皮犬种质资源的混乱状况提供了理论依据。

Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers

Ninety two blood samples of Chinese Shar-pei (CSP) dog, including 21 bone-mouth type, 29 bone-meat-mouth type and 42 meat-mouth type, were analysed by using 20 microsatellite loci. The results showed that a total of 272 alleles were found, averaging 13.6 alleles per locus. The allele frequencies of each type of CSP dog were calculated, showing some genetic differentiation among the three types of CSP. The average heterozygosity of the whole populaiton was 0.8259, and the polymorphism information content (PIC) of the whole CSP populaiton was between 0.5352 and 0.9226, indicating that the microsatellite loci are highly polymorphic. The dendrogram constructed from genetic distance showed that there was some divergence between the three CSP dog populations, which would support the theoretical basis for further subclassificaion to solve the disorganized situation of this breed and provide scientific guidance for better protectioin of this breed.