Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli |
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Authors: | Christine Martin Brigitte Cami Françoise Borne Davis J Jeenes Dieter Haas and Jean-Claude Patte |
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Institution: | (1) Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, 31, Chemin J. Aiguier, F-13277 Marseille Cedex 9, France;(2) Mikrobiologisches Insitut, Eidgenossische Technische Hochschule Zurich, Ch-8092 Zurich, Switzerland |
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Abstract: | Summary Chlorsulfuron-resistant mutants of Arabidopsis thaliana were isolated by screening for growth of seedlings in the presence of the herbicide. Both whole plants and derived tissue cultures were resistant to concentrations of the herbicide approximately 300-fold higher than that required to prevent growth of the wild-type. The resistance is due to a single dominant nuclear mutation at a locus designated csr which has been genetically mapped to chromosome-3. Acetohydroxy acid synthase activity in extracts from chlorsulfuron-resistant plants was much less-susceptible to inhibition by chlorsulfuron and a structurally related inhibitor than the activity in wild-type extracts. This suggests that the csr locus is the structural gene for acetohydroxy acid synthase. |
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Keywords: | Pseudomonas lysA Heterologous expression |
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