DNMT1 and HDAC1 gene expression in impaired spermatogenesis and testicular cancer |
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Authors: | Olufunmilade A Omisanjo Katharina Biermann Sonja Hartmann Lukas C Heukamp Violetta Sonnack Anne Hild Ralph Brehm Martin Bergmann Wolfgang Weidner Klaus Steger |
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Institution: | (1) Department of Urology and Pediatric Urology, Giessen, Germany;(2) Institute of Pathology, Bonn, Germany;(3) Institute of Veterinary Anatomy, Histology and Embryology, Giessen, Germany;(4) Klinik und Poliklinik für Urologie und Kinderurologie, Rudolf-Buchheim-Strasse 7, 35385 Giessen, Germany |
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Abstract: | DNA methylation catalyzed by DNA methyltransferases (DNMTs) and histone deacetylation catalyzed by histone deacetylases (HDACs)
play an important role for the regulation of gene expression during carcinogenesis and spermatogenesis. We therefore studied
the cell-specific expression of DNMT1 and HDAC1 for the first time in human testicular cancer and impaired human spermatogenesis.
During normal spermatogenesis, DNMT1 and HDAC1 were colocalized in nuclei of spermatogonia. While HDAC1 was additionally present
in nuclei of Sertoli cells, DNMT1 was restricted to germ cells exhibiting a different expression pattern of mRNA (in pachytene
spermatocytes and round spermatids) and protein (in round spermatids). Interestingly, in infertile patients revealing round
spermatid maturation arrest, round spermatids lack DNMT1 protein, while pachytene spermatocytes became immunopositive for
DNMT1. In contrast, no changes in the expression pattern could be observed for HDAC1. This holds true also in testicular tumors,
where HDAC1 has been demonstrated in embryonal carcinoma, seminoma and teratoma. Interestingly, DNMT1 was not expressed in
seminoma, but upregulated in embryonal carcinoma.
Olufunmilade A. Omisanjo is a scholarship holder of the German Academic Exchange Service (DAAD). Sonja Hartmann is a member
of the German Research Foundation (DFG) Research Training Group 533 Cell–cell-Interaction in Reproduction. |
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Keywords: | DNMT1 HDAC1 Spermatogenesis Testis Tumor |
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