The interaction of vinyl chloride with rat hepatic microsomal cytochrome P-450 in vitro. |
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Authors: | K M Ivanetich I Aronson I D Katz |
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Institution: | Department of Physiology and Medical Biochemistry University of Cape Town Medical School, Cape Town, South Africa |
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Abstract: | In the presence of hepatic microsomes, vinyl chloride produces a ‘type I’ difference spectrum and stimulates carbon monoxide inhibitable NADPH consumption. A comparison of the binding and Michaelis parameters for the interaction of vinyl chloride with uninduced, phenobarbital and 3-methylcholanthrene induced microsomes indicates that the binding and metabolism of vinyl chloride is catalyzed by more than one type P-450 cytochrome, but predominantly by cytochrome P-450. Metabolites of vinyl chloride from this enzyme system decrease the levels of cytochrome P-450 and microsomal heme, but not cytochrome 5 or NADPH-cytochrome reductase . |
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Keywords: | Con A Concanavalin A PAS periodic acid-Schiff PMSF phenylmethyl-sulfonylfluoride |
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