Presence of calmodulin-like calcium-binding protein in Bacillus cereus T spores |
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Authors: | Yuantay Shyu Peggy M. Foegeding |
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Affiliation: | Department of Food Science, North Carolina State University, Raleigh, North Carolina, U.S.A. |
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Abstract: | Abstract Bacillus cereus T spores were extensively washed, broken, and heated at 90°C for 2 min. Using calcium-dependent hydrophobic interaction chromatography, a single peak protein fraction was obtained which possessed calcium-binding capacity and some characteristics of calmodulin. This heat-stable protein fraction was retained by hydrophobic matrices (Phenyl-Sepharose) or a calmodulin antagonist (naphthalenesulfonamide) in a calcium-dependent manner. Calcium binding ability was verified by 45Ca autoradiography and a competitive calcium binding assay using Chelex-100. The crude spore extract displaced bovine brain calmodulin from its antibody in a radioimmunoassay and the immunoreactive specific activity of the partially purified fraction was approx. 200-fold greater than the crude spore extract. Thus, B. cereus T spores have a calcium-binding protein with calmodulin-like properties. |
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Keywords: | Calmodulin Calcium binding protein Bacillus cereus Bacterial spore |
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