| 人TRALL基因cDNA的克隆与序列测定 |
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| 引用本文： | 刘孟珉,萨其拉,鱼咏涛,贺福初.人TRALL基因cDNA的克隆与序列测定[J].生物技术通讯,1999(1). |
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| 作者姓名： | 刘孟珉 萨其拉 鱼咏涛 贺福初 |
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| 作者单位： | 北京放射医学研究所!北京100850 |
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| 摘 要： | 为研究人 TRALL的基因组结构 ,生物学性能和用于肿瘤生物治疗的可能性 ,利用反转录聚合酶链反应 (RT- PCR)从人急性早幼粒白血病细胞系 HL - 6 0细胞总 PNA中扩增出人 TRALL基因编码区 c DNA序列 ,将其克隆至 p GEM- T载体中 ,序列测定表明 ,克隆片段与文献报道的人TRALL基因编码区 c DNA序列完全一致。
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| 关 键 词： | TRALL 反转录聚合酶链反应 cDNA克隆 序列分析 |
Cloning and sequencing of human TRALL cDNA |
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| Liu Mengmin,Sa Qila,Yu Yongtao,He Fuchu.Cloning and sequencing of human TRALL cDNA[J].Letters in Biotechnology,1999(1). |
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| Authors: | Liu Mengmin Sa Qila Yu Yongtao He Fuchu |
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| Abstract: | For studing genomic structure, biological actions and potential utility as an anti-canceragent of human TRALL, human TRALL cDNA was amplified with total RNA from human acute promyelocytic leukemia cell line HL-60 by RT-PCR, then cloned into pGEM-T vector and sequenced. The cloned human TRALL cDNA sequence is identical to the published sequence. |
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| Keywords: | human TRALL RT-PCR cDNA cloning sequencing |
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