Characterization of specific binding of atrial natriuretic peptide (ANP) to rat PC12 phaeochromocytoma cells |
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Authors: | A Boumezrag F Lyall J A Dow |
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Institution: | MRC Blood Pressure Unit, Western Infirmary, Glasgow, Scotland, U.K. |
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Abstract: | Specific binding sites for atrial natriuretic peptide have been identified in membrane of the phaeochromocytoma cell line PC12. Scatchard analysis of binding studies revealed a Kd of 794 pM and a density (Bmax) of 254 fmol/mg protein. Hormones unrelated to ANP such as angiotensin II, bradykinin and arginine-8-vasopressin did not complete for the binding sites. Of the ANP-related peptides which competed for the binding sites, the following order of affinity was established; rANP (8-33) greater than rANP (28 amino acid) greater than rat atrial peptide fragment (13-28) greater than a-hANP (28 amino acid) greater than atrial peptide fragment (1-11) greater than atriopeptin I. |
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