Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy |
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Authors: | Stefania Marcheggiani Marcello Iaconelli Annamaria D'angelo Elio Pierdominici Giuseppina La Rosa Michele Muscillo Michele Equestre Laura Mancini |
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Institution: | (1) Department of Environment and Primary Prevention, Istituto Superiore di Sanit, Viale Regina Elena 299, 00169 Rome, Italy;(2) Department of Cell Biology and Neurosciences, Istituto Superiore di Sanit, Viale Regina Elena 299, 00169 Rome, Italy |
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Abstract: | Background Microbiological indicators are commonly used in the assessment of public health risks associated with fecal contamination
of freshwater ecosystems. Sediments are a reservoir of microorganisms, and can thus provide information on past pollution
events, not obtainable through the testing of surface water. Moreover, pathogens present in sediment may represent future
threats to human health. Clostridium perfringens, a typical colonizer of sediments, has been suggested as an alternative indicator of fecal pollution. In order to be suitable
for such purpose, the microorganism should be widely distributed in contaminated environments. The objective of this study
was thus to determine the composition of the anaerobic community in sediment samples of the lower Tiber basin, in central
Italy, through a combined approach involving granulometric analysis of sediment samples, as well as a microbiological and
molecular (16S rRNA) analysis of strains. |
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