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Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy
Authors:Stefania Marcheggiani  Marcello Iaconelli  Annamaria D'angelo  Elio Pierdominici  Giuseppina La Rosa  Michele Muscillo  Michele Equestre  Laura Mancini
Institution:(1) Department of Environment and Primary Prevention, Istituto Superiore di Sanit, Viale Regina Elena 299, 00169 Rome, Italy;(2) Department of Cell Biology and Neurosciences, Istituto Superiore di Sanit, Viale Regina Elena 299, 00169 Rome, Italy
Abstract:

Background  

Microbiological indicators are commonly used in the assessment of public health risks associated with fecal contamination of freshwater ecosystems. Sediments are a reservoir of microorganisms, and can thus provide information on past pollution events, not obtainable through the testing of surface water. Moreover, pathogens present in sediment may represent future threats to human health. Clostridium perfringens, a typical colonizer of sediments, has been suggested as an alternative indicator of fecal pollution. In order to be suitable for such purpose, the microorganism should be widely distributed in contaminated environments. The objective of this study was thus to determine the composition of the anaerobic community in sediment samples of the lower Tiber basin, in central Italy, through a combined approach involving granulometric analysis of sediment samples, as well as a microbiological and molecular (16S rRNA) analysis of strains.
Keywords:
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