Ethanol directly increases dihydrotestosterone conversion to 5 alpha-androstan-3 beta,17 beta-diol and 5 alpha-androstan-3 alpha,17 beta-diol in rat Leydig cells |
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Authors: | E P Murono V Fisher-Simpson |
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Institution: | 1. Medical Research Service, Wm. Jennings Bryan Dorn Veterans'' Hospital, Columbia, South Carolina 29201 USA;2. Department of Medicine, University of South Carolina School of Medicine, Columbia, South Carolina 29201 USA;3. Department of Physiology, University of South Carolina School of Medicine, Columbia, South Carolina 29201 USA |
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Abstract: | The direct effect of ethanol on dihydrotestosterone (DHT) conversion to 5 alpha-androstan-3 beta,17 beta-diol (3 beta-diol) and 5 alpha-androstan-3 alpha,17 beta-diol (3 alpha-diol) by adult rat Leydig cells was examined. Concentrations of ethanol comparable to blood levels of alcoholic men (2.2 - 65 mM) increased DHT conversion to 3 beta - and 3 alpha-diol, in direct relation to the dose of ethanol added; a 2-fold or greater stimulation was observed. Because this effect was blocked by 4-methylpyrazole or a saturating NADH concentration, these results suggest that this action is mediated by Leydig cell alcohol dehydrogenase activity. These results may have significant impact in the testis and/or other DHT sensitive tissues because ethanol may decrease the availability of the proposed active androgen. |
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Keywords: | DHT 5α-androstan-17β-ol-3-one 3α-diol 5α-androstan-3α 17βdiol 3β-diol 5α-androstan-3β 17β-diol 5α-ane-3α-HSD 5α-androstane-3α-hydroxysteroid dehydrogenase 5α-ane-3β-HSD 5α-androstane-3β-hydroxysteroid dehydrogenase Med 199-BSA Medium 199 containing 0 1% bovine serum albumin |
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