Effects of farnesoid X receptor on the expression of the fatty acid synthetase and hepatic lipase |
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Authors: | Li-Li Shen Hong Liu Jiahe peng Lin Gan Li Lu Qian Zhang Liangpeng Li Fengtian He Yu Jiang |
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Institution: | (1) Department of Biochemistry and Molecular Biology, The Third Military Medical University, Chongqing, 400038, China;(2) Department of Hematology, Xin-Qiao Hospital, Third Military Medical University, Chongqing, 400037, China; |
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Abstract: | The farnesoid X receptor (FXR) is a nuclear receptor that regulates gene expression in response to bile acids (BAs). FXR plays
an important role in the homeostasis of bile acid, cholesterol, lipoprotein and triglyceride. In this report, we identified
fatty acid synthase (FAS) and hepatic lipase (HL) genes as novel target genes of FXR. Human hepatoma HepG2 cells were treated
with chenodeoxycholic acid, the natural FXR ligand, and the messenger RNA and protein levels of FAS and HL were determined
by RT-PCR and Western blot analysis, respectively. Chenodeoxycholic acid (CDCA) down-regulated the expression of FAS and HL
genes in a dose and time-dependent manner in human hepatoma HepG2 cells. In addition, treatment of mice with CDCA significantly
decreased the expression of FAS and HL in mouse liver and the activity of HL. These results demonstrated that FAS and HL might
be FXR-regulated genes in liver cells. In view of the role of FAS and HL in lipogenesis and plasma lipoprotein metabolism,
our results further support the central role of FXR in the homeostasis of fatty acid and lipid. |
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