Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression |
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| Authors: | Heleen Nailis Soňa Kucharíková Markéta Řičicová Patrick Van Dijck Dieter Deforce Hans Nelis Tom Coenye |
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| Affiliation: | (1) Laboratory for Pharmaceutical Microbiology, Universiteit Gent, Harelbekestraat 72, B-9000 Ghent, Belgium;(2) Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium;(3) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium;(4) Department of Microbiology and Virology, Comenius University in Bratislava, Faculty of Natural Sciences, Mlynska dolina B-2, 842 15 Bratislava, Slovakia;(5) Laboratory for Pharmaceutical Biotechnology, Universiteit Gent, Harelbekestraat 72, B-9000 Ghent, Belgium |
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| Abstract: | Background Candida albicans infections are often associated with biofilm formation. Previous work demonstrated that the expression of HWP1 (hyphal wall protein) and of genes belonging to the ALS (agglutinin-like sequence), SAP (secreted aspartyl protease), PLB (phospholipase B) and LIP (lipase) gene families is associated with biofilm growth on mucosal surfaces. We investigated using real-time PCR whether genes encoding potential virulence factors are also highly expressed in biofilms associated with abiotic surfaces. For this, C. albicans biofilms were grown on silicone in microtiter plates (MTP) or in the Centres for Disease Control (CDC) reactor, on polyurethane in an in vivo subcutaneous catheter rat (SCR) model, and on mucosal surfaces in the reconstituted human epithelium (RHE) model. |
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