Surveillance of pyrazinamide susceptibility among multidrug-resistant <Emphasis Type="Italic">Mycobacterium tuberculosis</Emphasis> isolates from Siriraj Hospital,Thailand |
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| Authors: | Jirarut Jonmalung Therdsak Prammananan Manoon Leechawengwongs Angkana Chaiprasert |
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| Institution: | (1) Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700 Bangkok, Thailand;(2) National Center for Genetic Engineering and Biotechnology, National Science and Technology, Development Agency, Ministry of Science and Technology, 12120 Pathumthani, Thailand;(3) Vichaiyut Hospital, Setsiri Road, 10400 Bangkok, Thailand;(4) Drug-Resistant Tuberculosis Research Fund, Siriraj Foundation, 10700 Bangkok, Thailand |
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| Abstract: | Background Susceptibility testing of pyrazinamide (PZA) against Mycobacterium tuberculosis is difficult to perform because the acidity of culture medium that is required for drug activity also inhibits the growth
of bacteria. In Thailand, very limited information has been generated on PZA resistance, particularly among multidrug-resistant
tuberculosis (MDR-TB) isolated from Thailand. Only two studies on PZA susceptibility among Thai M. tuberculosis strains have been reported; one used a pyrazinamidase assay, and the other used the BACTEC 460 TB for PZA susceptibility
testing. In this study, we determined the percentage of strains possessing pyrazinamide resistance among pan-susceptible M. tuberculosis and MDR-TB isolates by using the pyrazinamidase assay, BACTEC MGIT 960 PZA method and pncA sequencing, and assessed the correlation in the data generated using these methods. The type and frequency of mutations in
pncA were also determined. |
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