| 血清学检测H-Y噬菌体Fab抗体阳性克隆的分析 |
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| 引用本文： | 王乃东,袁安文,邓治邦,许道军,马君,薛立群. 血清学检测H-Y噬菌体Fab抗体阳性克隆的分析[J]. 中国实验动物学报, 2011, 19(5): 387-390,I0003. DOI: 10.3969/j.issn.1005-4847.2011.05.006 |
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| 作者姓名： | 王乃东 袁安文 邓治邦 许道军 马君 薛立群 |
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| 作者单位： | 湖南农业大学动物医学院,长沙,410128 |
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| 基金项目： | 国家自然科学基金，湖南省教育厅科学研究优秀青年项目，湖南省研究生创新基金 |
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| 摘 要： | 目的 为分析H-Y噬菌体Fab抗体特异性,筛选用于抗体亲和力提高的H-Y噬菌体Fab抗体阳性克隆.方法 以从噬菌体Fab抗体库中筛选到具有雄性特异性结合活性的阳性克隆A6、A8、E6为基础,通过C57BL/6鼠脾细胞为抗原的ELISA分析3株阳性克隆的特异性,镜下观察亲和力较好的A8阳性克隆ELISA结果,利用生物信息学方法预测分析该克隆的抗体基因可变区序列和结构.结果 ELISA分析显示3株阳性克隆具有雄性特异性,其中A8阳性克隆具备较好的雄性特异性.A8克隆具有免疫球蛋白轻链和重链可变区结构,其重链、轻链可变区分别属于VHI和VκIV基因家族.结论 A8阳性克隆可用于后续的导向筛选和抗体基因改造等研究工作.
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| 关 键 词： | 小鼠 H-Y抗原 噬菌体展示技术 |
Analysis of positive clone of phage Fab antibody against serologically detected H-Y antigen |
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| WANG Nai-dong,YUAN An-wen,DENG Zhi-bang,XU Dao-jun,Ma Jun,XUE Li-qun. Analysis of positive clone of phage Fab antibody against serologically detected H-Y antigen[J]. Acta Laboratorium Animalis Scientia Sinica, 2011, 19(5): 387-390,I0003. DOI: 10.3969/j.issn.1005-4847.2011.05.006 |
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| Authors: | WANG Nai-dong YUAN An-wen DENG Zhi-bang XU Dao-jun Ma Jun XUE Li-qun |
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| Affiliation: | WANG Nai-dong,YUAN An-wen,DENG Zhi-bang,XU Dao-jun,Ma Jun,XUE Li-qun (College of Veterinary Medicine,Hunan Agriculture University,Changsha 410128,China) |
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| Abstract: | Objective In order to analyze the specificity of H-Y phage Fab antibody,positive clone of H-Y phage Fab antibody was screened to be used for improving the antibody affinity.Method Based on A6,A8,E6 positive clone with male specific activity screened from phage Fab antibody library,male specificity of 3 positive clones was tested by ELISA with male or female C57BL/6 mouse spleen cells.The result of ELISA for A8 positive clone was observed under microscope.Sequence and structure of variable region from positi... |
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| Keywords: | Mouse H-Y antigen Phage display technology |
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