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| 人骨髓间充质干细胞脑内移植对食蟹猴脑出血的治疗作用 | |
| 引用本文： | 朱华,冯铭,卢珊,李秦,刘颖,陈云新,代小伟,李昊,孙井江,安沂华,赵春华,王任直,秦川. 人骨髓间充质干细胞脑内移植对食蟹猴脑出血的治疗作用[J]. 中国实验动物学报, 2011, 19(5): 361-365,I0001,I0002. DOI: 10.3969/j.issn.1005-4847.2011.05.001 |
| 作者姓名： | 朱华 冯铭 卢珊 李秦 刘颖 陈云新 代小伟 李昊 孙井江 安沂华 赵春华 王任直 秦川 |
| 作者单位： | 1. 中国医学科学院;北京协和医学院比较医学中心;国家中医药管理局人类疾病动物模型三级实验室 2. 中国医学科学院北京协和医院,北京,1000730 3. 中国医学科学院医学实验动物研究所Motac合作实验室,北京,100021 4. 中国医学科学院基础医学研究所,北京,1000055 5. 北京神经外科研究所,北京,100039 |
| 摘 要： | 目的评价人骨髓间充质干细胞脑内移植对食蟹猴脑出血模型的治疗作用。方法符合普通级标准的成年食蟹猴12只,用自体股动脉抗凝血脑内注射方法建模后1周,用脑立体定位法在血肿周围植入人骨髓间充质干细胞,细胞数分别为高剂量5×106、低剂量1×106、对照组等体积生理盐水。利用MRI、PET、神经功能缺损评分和组织病理学对干细胞移植效果进行评价。结果神经功能评分显示干细胞移植1周后动物神经功能明显改善。PET结果显示干细胞移植后2周高剂量组血肿周围皮层、基底节核团的SUV%值与对照组间存在显著性差异(P=0.02)。移植后3周高、低剂量组血肿周围皮层、基底节核团的SUV%值与对照组间差异存在显著性(P值分别为0.03和0.04)。MRI显示剂量组血肿吸收速度大于对照组。病理检查可见剂量组坏死灶面积小于对照组,出血灶周围有大量新生血管生成,剂量组与对照组间差异存在显著性(P<0.01)。结论在损伤脑组织周围移植hBMSC可促进食蟹猴损伤神经组织的恢复,为hBMSC治疗脑出血的临床应用提供了重要实验依据。 |
| 关 键 词： | 间充质干细胞 脑出血 移植 食蟹猴 |
Intracerebral transplantation of human bone marrow mesenchymal stem cells promotes the functional recovery in Macaca fascicularis with intracerebral hemorrhage |
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| ZHU Hua,FENG Ming,LU Shan,LI Qin,LIU Ying,CHEN Yun-xin,DAI Xiao-wei,LI Hao,SUN Jing-jiang,AN Yi-hua,ZHAO Chun-hua,WANG Reng-zhi,QIN Chuan. Intracerebral transplantation of human bone marrow mesenchymal stem cells promotes the functional recovery in Macaca fascicularis with intracerebral hemorrhage[J]. Acta Laboratorium Animalis Scientia Sinica, 2011, 19(5): 361-365,I0001,I0002. DOI: 10.3969/j.issn.1005-4847.2011.05.001 | |
| Authors: | ZHU Hua FENG Ming LU Shan LI Qin LIU Ying CHEN Yun-xin DAI Xiao-wei LI Hao SUN Jing-jiang AN Yi-hua ZHAO Chun-hua WANG Reng-zhi QIN Chuan |
| Affiliation: | ZHU Hua1,FENG Ming3,LU Shan4,LI Qin2,LIU Ying1,CHEN Yun-xin1,DAI Xiao-wei1,LI Hao2,SUN Jing-jiang1,AN Yi-hua5,ZHAO Chun-hua4,WANG Reng-zhi3,QIN Chuan1(1.Center of Comparative Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine,Beijing 100021,China,2.Motac Laboratory,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences2,3.Peking Union Medical Col... |
| Abstract: | Objective To investigate the therapeutic effect of human bone marrow-derived mesenchymal stem cell(hBMSC) transplantation on experimentally induced intracerebral hemorrhage in Macaca fascicularis,and to examine the effects of hBMSC transplantation on the recovery of neurological function.Methods In 12 Macaca fascicularis,autologous femoral arterial blood was injected into the brain parenchyma to develop an intracerebral hemorrhage(ICH) model.The animals were randomly divided into high-dose,low-dose,and cont... |
| Keywords: | Bone marrow mesenchymal stem cells Intracerebral hemorrhage Transplantation Macaca fascicularis |
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