Continuous production of the lipopeptide biosurfactant of Bacillus licheniformis JF-2 |
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Authors: | S -C Lin K S Carswell M M Sharma G Georgiou |
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Institution: | (1) Department of Chemical Engineering, The University of Texas at Austin, 78712 Austin, TX, USA;(2) Department of Petroleum Engineering, The University of Texas at Austin, 78712 Austin, TX, USA |
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Abstract: | Bacillus licheniformis JF-2 synthesizes a surfactin-like lipopeptide that is the most effective biosurfactant known. In shake-flask cultures the biosurfactant is produced by actively growing cells (mid-linear phase), but subsequently it becomes rapidly internalized by the cells as soon as the culture enters the stationary phase. This deactivation phenomenon is a major hurdle in the efficient production of the biosurfactant. We have shown that the synthesis of the JF-2 lipopeptide is strongly dependent on O2 concentration with substantial production observed only in cultures grown under O2-limiting conditions. In continuous cultures the biosurfactant was produced only within a narrow window of low dilution rates. At a dilution rate of 0.12 h–1 and low dissolved O2, the biosurfactant concentration was maintained at 33 mg/l, which is virtually the same as the maximum concentration obtained in optimized batch fermentations. |
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