不同生长底物对培养背根神经节细胞的影响(简报)

发育期细胞和细胞外基质(extracellular matrix,ECM)之间的相互作用调节着细胞的功能,包括细胞的迁移、细胞骨架的构建、细胞的增值和分化。神经元“移居”体外后,失去了在体内所依托的组织学关系,必须黏附于一个固相表面才能生存,所以神经元只有在包被基质的培养器皿上才能存活,对于分离的神经元来说,能否尽快粘附到生长基质上是影响神经元体外存活的因素之一。许多研究证明

INFLUENCE OF SUBSTRATES COATED ON CULTURED DORSAL ROOT GANGLION CELLS

Dorsal tool ganglia from rat embryos were digested with trypsin to form single cellsuspension.The cells were seeded on culture plates coated respectingly with poly-L-lysin (PLL),laminin(LN),PLL combined with LN,or collagen type 1 (Col),then cultured in NBI media.Thecell survival and neurite outgrowth of cultured dorsal rool ganglion cells were observed by phase-contrast microscopy.The results showed that the dorsal root ganglion neurons grown on PLL com-bined with LN were dispersively distributed at high survival rate.and those cells grown on Colwere clustered with thicker and longer neurites.It is suggested that the cell growth pattern couldbe influeuced by different substances coated on the plates,and PLL combined with LN may pro-vide a better substrum to culture and study single neuron soma and neurite.