Down-regulation of apoptosis-inducing factor protein by RNA interference inhibits UVA-induced cell death |
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Authors: | Yuan Chang-Qing Li Ya-Na Zhang Xu-Feng |
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Affiliation: | Laboratory of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China. yuanchq@hotmail.com |
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Abstract: | Apoptosis-inducing factor (AIF) is a caspase-independent apoptosis effector. UVA-induced Raji cell death was not completely inhibited by pan-caspase inhibitor zVAD.fmk. Moreover, AIF translocated from its normal location, the mitochondrial intermembrane space, into the nucleus, and induced peripheral chromatin condensation during the early stage of UVA-inducing cell death. Enforced expression of AIF can induce Raji cell death in a caspase-independent manner. Down-regulation of AIF protein level by RNA interference (RNAi) can reduce UVA-induced Raji cell death, but the combination of down-regulation of AIF and zVAD.fmk almost completely inhibited UVA-induced Raji cell death. All these suggest that caspase and AIF are two independent pathways and that UVA-induced Raji cell death is dependent on caspase and AIF. |
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Keywords: | Apoptosis-inducing factor RNA interference UVA Apoptosis |
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