Proteolytic activities and ribulose 1,5 bisphosphate carboxlyase degradation in leaves of soybean (Glycine max L. Merril) with different nitrogen status

Changes in soluble proteins and Rubisco (E.C.4.1.1.39) contents were examined in leaves of nitrogen-deprived and nitrogen-sufficient soybeans. Rubisco content was very responsive to nitrogen stress, and this protein appeared to be the largest source of mobilizable nitrogen in the senescent leaf. Loss of soluble proteins and Rubisco was associated with a decrease in the activities of several proteolytic enzymes measured using artificial substrates: carboxypeptidase, aminopeptidase and haemoglobinase.The in vitro activity of enzyme(s) which can degrade Rubisco was investigated using endogenous Rubisco and in vitro radiolabelled Rubisco as substrates. Highest endopeptidic cleavage of endogenous Rubisco occured at pH 4; the enzyme responsible for this breakdown appeared to be a sulfhydryl-dependent proteinase. In contrast, [¹⁴C] Rubisco was attacked preferentially at pH 9, by a peptide hydrolase sensitive to EDTA. No increase in Rubisco-degrading activities was detected in nitrogen-deficient soybean leaves compared to control plant leaves.Abbreviations EDTA Ethylenediaminetetraacetate - LS Large Subunit of Rubisco - NEM N-ethylmaleimide - pCMB Parachloromercuribenzoate - PMSF Phenylmethylsulfonyl-fluoride - Rubisco Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase