| 双抗体免疫沉淀法纯化牛生长激素poly(A)~+RNA的研究 |
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| 引用本文： | 史瀛仙,宋德秀,戴宗汉. 双抗体免疫沉淀法纯化牛生长激素poly(A)~+RNA的研究[J]. 中国生物化学与分子生物学报, 1989, 5(4): 354-358 |
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| 作者姓名： | 史瀛仙 宋德秀 戴宗汉 |
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| 作者单位： | 中国科学院发育生物学研究所 北京(史瀛仙，宋德秀)，中国科学院发育生物学研究所 北京(戴宗汉) |
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| 摘 要： | 我们用双抗体免疫沉淀法,从牛垂体多聚核糖体中分离出牛生长激素特异多聚核糖体,由此多聚核糖体纯化的牛生长激素Poly(A)~+RNA,可以在麦胚体外翻译系统中和兔网织红细胞体外翻译系统中促进~(14)C-亮氨酸的参入。合成的含~(14)-亮氨酸的翻译产物中有91％可以被牛生长激素抗体沉淀。用SDS-11％PAGE对翻译产物进行鉴定表明,翻译产物在25KD处呈一条放射自显影带,与报导的牛生长激素前体分子量相吻合。
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| 关 键 词： | 牛生长激素 poly(A)~+RNA 免疫沉淀 产物鉴定 |
| 收稿时间： | 1989-08-20 |
A Study on the Isolation and Purification of Bovine Growth Hormone Poly(A)~+RNA by Immunoprecipitation |
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| Shi,Ying-xian. A Study on the Isolation and Purification of Bovine Growth Hormone Poly(A)~+RNA by Immunoprecipitation[J]. Chinese Journal of Biochemistry and Molecular Biology, 1989, 5(4): 354-358 |
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| Authors: | Shi Ying-xian |
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| Affiliation: | (Ying-hsien Shih) Song, De-xiu Dai, Zhong-han (Institute of Developmental Biology, Academia Sinica, Beijing |
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| Abstract: | A procedure for isolation and purification of bovine growth hormone poly(A) RNA by immunoprecipitation is described. The bovine growth hormone poly(A) RNA purified from bovine pituitary can stimulate the incorporation of 14C-Leucine into protein in wheat germ cell-free system and rabbit reticuloeyte system. The translation product of bGH poly(A) RNA corresponds to the bGH precursor. The purity of bGH poly(A) RNA is about 91%. Denatured agrose gel (1%) electrophoresis of the poly (A) RNA exhibits one band which is approximately 11S. |
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| Keywords: | Bovine growth hormone Poly(A)~ RNA Immunoprecipitation Identification of translation product |
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