The lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells |
| |
| Authors: | G M S Van Slogteren P J J Hooykaas K Planqué B De Groot |
| |
| Institution: | (1) J.A. Cohen Institute for Radiopathology and Radiation Protection, Leiden;(2) Department of Biochemistry, Leiden, The Netherlands;(3) Dept. of Biochemistry, Wassenaarsweg 64, 2333 AL Leiden, The Netherlands;(4) Present address: ITAL, Keyenbergsweg 6, Wageningen, The Netherlands |
| |
| Abstract: | Plant cells transformed into octopine-synthesizing tumour cells by the bacterium Agrobacterium tumefaciens survive when cultured in the presence of homo-arginine (HA), whereas both normal plant cells and nopaline producing plant tumour cells do not. Survival of octopine crown gall cells is due to the activity of the enzyme lysopinedehydrogenase (LpDH) in these cells, which converts toxic homo-arginine into non-toxic homo-octopine. The selective toxicity of homo-arginine for normal cells can be applied for the enrichment of octopine Ti plasmid transformed plant cells vs normal plant cells in mixed cultures. |
| |
| Keywords: | Agrobacterium tumefaciens crown gall lysopinedehydrogenase Ti plasmid homo-arginine plant vectors |
| 本文献已被 SpringerLink 等数据库收录！ |
|
