Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977 |
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| Authors: | Moriya Tatsuki Watanabe Manabu Sumida Naomi Okakura Kaoru Murakami Takeshi |
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| Institution: | Microbiological Resources and Technology Laboratories, Meiji Seika Kaisya Ltd., 788 Kayama, Odawara-shi 250-0852, Japan. tatsuki_moriya@meiji.co.jp |
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| Abstract: | The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain. |
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