Transfer of phospholipids from microsomes to mitochondria in germinating castor bean endosperm |
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Authors: | Yamada Mitsuhiro; Tanaka Toshinori; Kader J C; Mazliak Paul |
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Institution: | 1Department of Biology, College of General Education, University of Tokyo Komaba, Meguro, Tokyo 153, Japan
2Laboratoire de Physiologie Cellulaire, Universit? de Paris VI 12, rue Cuvier, 75005 Paris, France |
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Abstract: | 14C-labeled microsomes were prepared by feeding 1-14 C]acetateto endosperm tissues from 4-day-old seedlings of castor beanseeds and incubated with unlabeled mitochondria from the sametissues. The loss of 14C-lipids from the microsomes was accompaniedby an increase of 14C-lipids in the mitochondria. The additionof 105,000?g supernatant and also pH 5.1-treated supernatant,both of which had been prepared from castor bean endospermsat the same stage, markedly enhanced the lipid transfer frommicrosomes to mitochondria. The activity in this fraction wasprecipitated by ammonium sulfate and lost with trypsin or heattreatment. The transfer of lipids was limited to phospholipids.Thus, it is concluded that in castor bean endosperms, phospholipidsare transferred from the endoplasmic reticulum to the mitochondriaby a phospholipid-exchange protein contained in the cytosol. (Received August 8, 1977; ) |
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