Genetic diversity and gene flow in a Caribbean tree <Emphasis Type="Italic">Pterocarpus officinalis</Emphasis> Jacq.: a study based on chloroplast and nuclear microsatellites |
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Authors: | F Muller M Voccia A Bâ J -M Bouvet |
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Institution: | Biological System Department, CIRAD, Campus international de Baillarguet TA-A 39/C, 34398, Montpellier cedex 5, France. felix.muller@laposte.net |
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Abstract: | We analysed the molecular diversity of Pterocarpus officinalis, a tree species distributed in Caribbean islands, South and Central America to quantify the genetic variation within island,
to assess the pattern of differentiation and infer levels of gene flow; with the overall goal of defining a strategy of conservation.
Two hundred two individuals of 9 populations were analysed using three chloroplast and six nuclear microsatellite markers.
The observed heterozygosity varied markedly among the populations for nuclear (H
Onuc = 0.20–0.50) and chloroplast microsatellites (H
cp = 0.22–0.68). The continental population from French Guyana showed a higher value of H
Onuc than island populations, and the differences were significant in some cases. The fixation index F
IS ranged from −0.043 to 0.368; a significant heterozygote deficit was detected in 7 populations. The heterozygosity excess
method suggested that two populations in Guadeloupe have undergone a recent bottleneck. Global and pairwise F
ST were high for both nuclear (F
STnuc = 0.29) and chloroplast microsatellites (F
STcp = 0.58). The neighbour-joining tree based on both markers, presented a differentiation pattern that can be explained by the
seed dispersal by flotation and marine stream. The comparison of Bayesian approach and the method based on allelic frequency
demonstrate a very limited number of migrants between populations. |
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Keywords: | Pterocarpus officinalis Jacq Nuclear microsatellites Chloroplast microsatellites Bayesian clustering Genetic structure Migrants |
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