Ac/Ds标签系统与水稻功能基因组学

自2002年水稻基因组测序完成后,水稻功能基因组学研究正在成为水稻研究的重要内容.构建突变体库是研究功能基因组学的一条重要而有效的途径.利用外源的Ac/Ds (Activator/Dissociation)标签系统是构建插入突变体库较为理想的方法,经过多年发展完善,其在水稻中已有广泛的应用,但仍面临着一些需要解决的实际问题.文章对Ac/Ds标签系统的转座行为及其构建突变体库的问题和优点进行了综述,总结了近年来Ac/Ds标签系统在水稻中的研究进展,分析了利用Ac/Ds标签系统进行功能基因组学研究所面临的挑战.

Ac/Ds Tagging System and Functional Genomics in Rice

With the completion of the rice ge- nome sequence in 2002, the study on functional genomics in rice has become a major task. Es- tablishment of rice mutant library is an essential approach for rice functional genomics study. At present, utilizing maize transposable element Ac/ Ds (Activator/Dissociation) is a promising method to construct insertional mutagenesis li- brary of rice. Ac/Ds tagging system has received extensive application in rice during the past sev- eral years, but it is still confronted with practical problems. In this paper, constructing rice inser- tional mutagenesis library using Ac/Ds-tagging system and transpositional behaviors of an Ac/Ds-tagging system, difficulties and advantages are reviewed. The research advances and chal- lenge in rice functional genomics study using Ac/ Ds tagging system are also discussed and sum- marized in the paper.