QTLs for a component of partial resistance to cucumber mosaic virus in pepper: restriction of virus installation in host-cells |
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Authors: | C Caranta A Palloix V Lefebvre A M Daubèze |
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Institution: | Institut National de la Recherche Agronomique (INRA), Station d’Amélioration des Plantes Mara?chères, BP 94, 84143 Montfavet Cedex, France, FR
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Abstract: | Ninety four doubled-haploid (DH) lines obtained from the F1 between Perennial, a cucumber mosaic virus (CMV)-partially resistant Capsicum annuum line, and Yolo Wonder, a CMV-susceptible C. annuum line, were analysed with 138 markers including mostly RFLPs and RAPDs. Clustering of RAPD markers was observed on five linkage
groups of the intraspecific linkage map. These clusters could correspond to the centromeric regions of pepper chromosomes.
The same progenies were evaluated for restriction of CMV installation in pepper cells in order to map quantitative trait loci
(QTLs) controlling CMV resistance. This component of partial resistance to CMV was quantitatively assessed using a CMV strain
that induced necrotic local lesions on the inoculated leaves. The number of local lesions gave an estimation of the density
of the virus-infection sites. Genotypic variance among the DH lines was highly significant for the number of local lesions,
and heritability was estimated to be 0.94. Using both analysis of variance and non-parametric tests, three genomic regions
significantly affecting CMV resistance were detected on chromosomes Noir, Pourpre and linkage group 3, together explaining
57% of the phenotypic variation. A digenic epistasis between one locus that controlled significant trait variation and a second
locus that by itself had no demonstrable effect on the trait was found to have an effect on CMV resistance. For each QTL,
the allele from Perennial was associated with an increased resistance. Implications of QTL mapping in marker-based breeding
for CMV resistance are discussed.
Received: 16 September 1996 |
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Keywords: | Capsicum annuumL Clustering of markers Cucumber mosaic virus Doubled-haploid progeny QTL mapping |
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