Diterpenoids and triterpenoids in hairy roots of Salvia sclarea |
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Authors: | Łukasz Kuźma Zuzanna Skrzypek Halina Wysokińska |
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Institution: | (1) Department of Biology and Pharmaceutical Botany, Medical University, Muszyńskiego 1, 90-151 Łódź, Poland |
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Abstract: | Hairy root culture of Salvia sclarea L. was established following infection with Agrobacterium rhizogenes LBA 9402. The culture was grown in growth regulator-free half-strength B5 Gamborg medium with 30 g l−1 sucrose and was investigated with respect to its capability of producing diterpenoids and triterpenoids. Four diterpenoids
(ferruginol, salvipisone, aethiopinone and 1-oxoaethiopinone) and two ursene-type triterpenoids (2α,3α-dihydroxy-urs-12-en-28-oic acid and 2α,3α,24-trihydroxy-urs-12-en-28-oic acid) were isolated from the hairy roots. The presence of three sterols (β-sitosterol, stigmasterol and campesterol), as well as oleanolic and ursolic acids was also shown by GC–MS analysis. The quantitative
and qualitative differences in diterpenoid and triterpenoid production patterns between hairy roots grown in the light and
in the dark were described. |
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Keywords: | aethiopinone Agrobacterium rhizogenes ferruginol hydroxy derivatives of ursolic acid 1-oxoaethiopinone salvipisone transformation |
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