Release of Carbonic Anhydrase from the Cell Surface of Chlamydomonas reinhardtii by Trypsin |
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Authors: | Yagawa, Yuriko Aizawa, Katsunori Yang, Shi-Yuan Miyachi, Shigetoh |
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Affiliation: | Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | Treatment with trypsin of Chlamydomonas reinhardtii cells grownin ordinary air (low-CO2 cells) caused almost complete releaseof carbonic anhydrase (CA) into the suspending medium, but didnot affect the shape and kinesis of the cells. These resultsindicate that most of the CA exists on the cell surface of low-CO2cells. The released CA has the same molecular weight, specificactivity and susceptibility to various CA inhibitors as thatpurified from non-treated low-CO2 cells. (Received August 24, 1985; Accepted November 20, 1985) |
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