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NADH: Nitrate reductase activity restoration by rhodanese
Authors:Umberto Tomati  Giovanni Giovannozzi-Sermanni  Silvestro Duprè  Carlo Cannella
Institution:1. Laboratory of Radiobiochemistry and Plant Ecophysiology, National Research Council, Rome, Italy;2. Institute of Biological Chemistry, University of Rome, Rome, Italy.
Abstract:The NADH: nitrate reductase from durum wheat leaves was inactivated by cyanide and its activity restored by thiosulphate and beef kidney rhodanese. Rhodanese and thiosulphate, added to NADH-nitrate reductase before cyanide treatment protected NADH-nitrate reductase activity. No oxidizing agent was required for the protection or restoration of cyanide treated NADH-nitrate reductase.
Keywords:Gramineae  durum wheat  NADH  nitrate reductase restoration  cyanide  rhodanese  
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