NADH: Nitrate reductase activity restoration by rhodanese |
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Authors: | Umberto Tomati Giovanni Giovannozzi-Sermanni Silvestro Duprè Carlo Cannella |
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Institution: | 1. Laboratory of Radiobiochemistry and Plant Ecophysiology, National Research Council, Rome, Italy;2. Institute of Biological Chemistry, University of Rome, Rome, Italy. |
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Abstract: | The NADH: nitrate reductase from durum wheat leaves was inactivated by cyanide and its activity restored by thiosulphate and beef kidney rhodanese. Rhodanese and thiosulphate, added to NADH-nitrate reductase before cyanide treatment protected NADH-nitrate reductase activity. No oxidizing agent was required for the protection or restoration of cyanide treated NADH-nitrate reductase. |
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Keywords: | Gramineae durum wheat NADH nitrate reductase restoration cyanide rhodanese |
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