Effect of ionic liquids activation on laccase from Trametes versicolor: Enzymatic stability and activity |
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| Authors: | Ana P M Tavares Juliana A N Pereira Ana M R B Xavier |
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| Institution: | 1. LSRE‐Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, , Porto, Portugal;2. Centre for Research in Ceramics and Composite Materials (CICECO), Department of Chemistry, University of Aveiro, , Aveiro, Portugal |
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| Abstract: | The stability and activity of laccase from Trametes versicolor in two water‐soluble ionic liquids (ILs), namely 1‐butyl‐3‐methylimidazolium methyl sulfate, bmim]MeSO4] and 1,3‐dimethylimidazolium methyl sulfate, mmim]MeSO4], were investigated in this study. Thermal inactivation of laccase was characterized in the presence of these both ILs and as expected first‐order kinetics was followed. Inactivation rate constant (k), half‐life time (t1/2), and energy of activation (Ea) were determined. Kinetics of 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) oxidation by laccase in the presence of these ILs was studied and Michaelis–Menten parameters were calculated. There is no enzymatic inactivation since the maximum reaction rate remained constant for IL concentrations up to 25%, and surprisingly, it was found that laccase was activated for concentrations of 35% of ILs, since the reaction rate increased 1.7 times. |
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| Keywords: | Activation of laccase Ionic liquids Michaelis– Menten kinetics Thermal inactivation Trametes versicolor |
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