Significance of caffeic acid-O-methyltransferase in lignification of cultured tobacco cells |
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| Authors: | Yasuyuki Yamada Toru Kuboi |
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| Affiliation: | Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan |
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| Abstract: | Caffeic acid-O-methyltransferase, activity was assayed in the callus and suspension cultured cells of tobacco. Lignification of cells was observed only in a kinetin (10?5 M) culture and not in an auxin (10?6 M 2,4-D or 10?5 M IBA) culture. Enzyme activity in the kinetin cultured cells was much higher than in the stock culture and the rise in enzyme activity coincided with the onset of lignification. |
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| Keywords: | Solanaceae tobacco callus and cell culture lignification kinetin. |
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