Photocontrol of chlorogenic acid biosynthesis in potato tuber discs |
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| Authors: | Christopher J. Lamb Philip H. Rubery |
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| Affiliation: | University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge, CB2 1QW, England |
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| Abstract: | The appearance of phenylalanine ammonia-lyase activity and the accumulation of chlorogenic acid in potato tuber discs are stimulated by illumination with white light, whereas the appearance of cinnamic acid 4-hydroxylase activity is unaffected by illumination. The photosensitive step in chlorogenic acid biosynthesis may be by-passed by treatment of discs with exogenous supplies of cinnamic acid, whereas treatment of discs with phenylalanine does not isolate the photosensitive step. Therefore, the site of photocontrol of chlorogenic acid biosynthesis in potato tuber discs is the reaction catalysed by phenylalanine ammonia-lyase. Cinnamic acid 4-hydroxylase activity in vitro is unaffected by p-coumaric acid, caffeic acid or chlorogenic acid. Phenylalanine ammonia-lyase activity in vitro is sensitive to inhibition by cinnamic acid. The in vitro properties of the two enzymes are also consistent with the hypothesis that phenylalanine ammonia-lyase rather than cinnamic acid 4-hydroxylase is important in the regulation of chlorogenic acid biosynthesis in potato tuber discs. |
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| Keywords: | Solanaceae potato biosynthesis polyphenols photocontrol chlorogenic acid phenylalanine ammonia-lyase cinnamic acid 4-hydroxylase. PAL: Phenylalanine ammonia-lyase CA4H: cinnamic acid 4-hydroxylase CGA: chlorogenic acid. |
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