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Intracellular distribution of the steroidal glycoalkaloid α-tomatine in Lycopersicon esculentum fruit
Authors:James G. Roddick
Affiliation:Department of Biological Sciences, University of Exeter, Exeter EX4 4QG, England
Abstract:Pericarp tissue from green tomato fruits was homogenized and separated into organelle fractions by differential centrifugation. Tomatine was found mainly in the final (105 000g) supernatant, with small amounts in the microsomes. Expressed sap from intact tissue was also rich in the alkaloid. It is suggested that tomatine accumulates in the vacuoles and/or soluble phase of the cytoplasm and is possibly synthesized in microsomal organelles.
Keywords:Solanaceae  tomato  intracellular distribution  steroidal glycoalkaloid  α-tomatine.
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