Different forms of EF1 and viability in wheat embryos |
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| Authors: | Antonio Dell&#x;Aquila Graziano Zocchi Gian Antonio Lanzani Piero De Leo |
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| Institution: | 1. Laboratorio del Germoplasma del C.N.R., Via Amendola, 165/A, 70126-Bari, Italy;2. lnstituto di Chimica Agraria dell''Università degli Studi di Milano, Via Celoria, 2-Milano, Italy |
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| Abstract: | Properties of heterogeneous forms of elongation factor (EF1) from cytoplasm of wheat embryos at different viability levels have been studied. The GTP-binding activity, the catalysis of the binding of Phe-14C]-tRNA to ribosomes and the complementation of EF2 for the synthesis of poly-U directed poly-Phe indicate that modifications of the EF1 activity occur as a result of seed ageing. The presence of a strong translational inhibitor was indicated in the cytoplasm of low viability embryos. |
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| Keywords: | wheat regulation of protein synthesis at translation level EF1 forms |
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