High‐level production of soluble pyrroloquinoline quinone‐dependent glucose dehydrogenase in Escherichia coli |
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| Authors: | Yi Yu Peilian Wei Xiangcheng Zhu Lei Huang Jin Cai Zhinan Xu |
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| Institution: | 1. Department of Chemical and Biological Engineering, Institute of Biological Engineering, Zhejiang University, , Hangzhou, P. R. China;2. Department of Biochemical Engineering, Zhejiang University of Science and Technology, , Hangzhou, P. R. China;3. Hunan Engineering Research Center of Combinatorial Biosynthesis and Natural Product Drug Discovery, , Changsha, Hunan, P. R. China |
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| Abstract: | Pyrroloquinoline quinone‐dependent glucose dehydrogenase (EC1.1.5.2, PQQGDH) has attracted progressive attention due to its application in glucose detection in clinic diagnosis and industrial bioprocess controls. To satisfy its increasing demand, improvement of PQQGDH production derived from Acinetobacter calcoaceticus L.M.D. 79.41 in recombinant Escherichia coli is necessary and is therefore the focus of the current study. Different carbon sources as well as induction conditions were investigated for overexpression of soluble PQQGDH. The results indicate that the target protein was optimally produced with 20 g/L glucose as the substrate. Moreover, the highest expression level (1530 kU/L) was achieved by a novel two‐temperature cultivation strategy in the 10‐L fermentor. This presents a sixfold improvement over previously reported values. After Ni‐NTA affinity chromatography purification, high‐purity enzyme with the specific activity of 5811 U/mg was obtained with a purification yield of 55%. The purified recombinant PQQGDH showed thermal stability and substrate specificity as the native enzyme. In summary, this work provides an alternative production process to overexpress PQQGDH and shows high applicability for large‐scale production of this important glucose dehydrogenase. |
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| Keywords: | Glucose dehydrogenase Optimization Recombinant expression PQQGDH |
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