Fructose-1,6-diphosphatase from Mangifera indica |
| |
Authors: | NN Rao VV Modi |
| |
Institution: | Department of Microbiology, M.S. University of Baroda, Baroda 390002, India |
| |
Abstract: | Fructose-1,6-diphosphatase (FDPase) from unripe mango was separated into two components by ammonium sulfate fractionation, one active at pH 6 (acidic FDPase) and the other at pH 8.5 (alkaline FDPase). The alkaline component had a lower Km. (0.15 × 10?3 M) than the acidic component (1.7 × 10?3 M) towards the substrate (FDP) and the allosteric inhibitor AMP. It also showed greater heat stability and higher activation in the presence of EDTA as compared to the acidic FDPase. Both components showed a higher activation with Mn2+ ions than with Mg2+ ions. |
| |
Keywords: | mangofruit fructose-1 6-diphosphatase properties and regulation |
本文献已被 ScienceDirect 等数据库收录! |
|