Synthesis of monoacylglycerol containing pinolenic acid via stepwise esterification using a cold active lipase |
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Authors: | Young‐Gil Pyo Seung In Hong Yangha Kim Byung Hee Kim In‐Hwan Kim |
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Institution: | 1. Dept. of Food and Nutrition, Korea University, Seoul, 136‐703, Republic of Korea;2. Dept. of Nutritional Science and Food Management, Ewha Womans University, Seoul, 120‐749, Republic of Korea;3. Dept. of Food Science and Technology, Chung‐Ang University, Anseong, 456‐756, Republic of KoreaByung Hee Kim and In‐Hwan Kim contributed equally to this research;4. Dept. of Food and Nutrition, Korea University, Seoul, 136‐703, Republic of KoreaByung Hee Kim and In‐Hwan Kim contributed equally to this research |
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Abstract: | High purity monoacylglycerol (MAG) containing pinolenic acid was synthesized via stepwise esterification of glycerol and fatty acids from pine nut oil using a cold active lipase from Penicillium camembertii as a biocatalyst. Effects of temperature, molar ratio, water content, enzyme loading, and vacuum on the synthesis of MAG by lipase‐catalyzed esterification of glycerol and fatty acid from pine nut oil were investigated. Diacylglycerol (DAG) as well as MAG increased significantly when temperature was increased from 20 to 40°C. At a molar ratio of 1:1, MAG content decreased because of the significant increase in DAG content. Water has a profound influence on both MAG and DAG content through the entire course of reaction. The reaction rate increased significantly as enzyme loading increased up to 600 units. Vacuum was an effective method to reduce DAG content. The optimum temperature, molar ratio, water content, enzyme loading, vacuum, and reaction time were 20°C, 1:5 (fatty acid to glycerol), 2%, 600 units, 5 torr, and 24 h, respectively. MAG content further increased via lipase‐catalyzed second step esterification at subzero temperature. P. camembertii lipase exhibited esterification activity up to ?30°C. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012 |
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Keywords: | diacylglycerol esterification glycerol monoacylglycerol Penicillium camembertii pinolenic acid |
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