Evaluation of the environmental conditions for the continuous production of lignin peroxidase by Phanerochaete Chrysosporium in fixed-bed bioreactors |
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| Authors: | S. Rodríguez Couto G. Feijoo M.T. Moreira J.M. Lema |
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| Affiliation: | (1) Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Avda. das Ciencias s/n, E-15706 Santiago de Compostela, Spain |
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| Abstract: | A new system to produce lignin peroxidase (LiP) continuously by Phanerochaete chrysosporium is described. A fixed-bed bioreactor with a pulsing device was used as the optimal bioreactor configuration. Addition of veratryl alcohol (1 mM), tryptophan (1 mM), no Mn2+ addition, low glucose addition rate (60–70 mg l–1 h) and an atmosphere of O2 gave maximum LiP activities of 700 U l–1, which are higher than those previously reported. |
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| Keywords: | continuous production fixed-bed bioreactor lignin peroxidase Phanerochaete chrysosporium pulsation system |
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