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PERMANENT GENETIC RESOURCES: Characterization of eight microsatellite loci in the woolly mouse opossum, Micoureus paraguayanus, isolated from Micoureus demerarae
Authors:Dias I M G  Amato G  Carvalho M R S  Cunha H M  Paglia A P  Desalle R  Fonseca C G
Institution:Departamentos de Biologia Geral e Zoologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-010, MG, Brazil, Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, 10024, NY, USA.
Abstract:Eight novel microsatellite markers were isolated from the woolly mouse opossum from the Amazon Forest in Peru, Micoureus demerarae, using a partial genomic DNA library and an enrichment protocol. These loci were polymorphic in M. demerarae and Micoureus paraguayanus populations from the Atlantic Forest in Brazil with the number of alleles ranging from two to 23. Those eight loci plus another five already described for M. paraguayanus will allow for the evaluation of genetic diversity of populations from the 'Rio Doce' Park, one of the last Atlantic Forest fragments in Minas Gerais state, Brazil.
Keywords:conservation  genomic library  Marsupialia  Micoureus  microsatellites  opossum
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