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PERMANENT GENETIC RESOURCES: Twelve new microsatellite markers for the Chinese shrimp Fenneropenaeus chinensis
Authors:Gao Huan  Kong Jie  Yan Binlun  Yu Fei  Luan Sheng  Cai Shengli
Institution:Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China, Shanghai Fisheries University, Shanghai 200090, China.
Abstract:Twelve new microsatellite markers were isolated by sequencing random clones from a genomic library of Fenneropenaeus chinensis. The number of alleles per locus ranged from five to 15. The polymorphism information content ranged from 0.568 to 0.898, and observed and expected heterozygosities from 0.344 to 0.882 and from 0.691 to 0.915, respectively. All loci except one conformed to Hardy-Weinberg equilibrium. These markers, described here for Chinese shrimp, will be further used to analyse the species' population genetics.
Keywords:alleles  Chinese shrimp  Fenneropenaeus chinensis  microsatellites  primer pairs  sequencing random clones
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