PERMANENT GENETIC RESOURCES: Isolation and characterization of 10 tetranucleotide microsatellite loci in an enigmatic East African bird, the spot-throat (Modulatrix stictigula) |
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| Authors: | Sellas Anna B Feldheim Kevin A Bowie Rauri C K |
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| Institution: | Museum of Vertebrate Zoology and Department of Integrative Biology, 3101 Valley Life Science Building, University of California, Berkeley, CA 94720, USA, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S Lake Shore Drive, Chicago, IL 60605, USA. |
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| Abstract: | We describe the isolation of 10 tetranucleotide microsatellites from the spot-throat using an enrichment protocol. All loci were highly variable with the number of alleles ranging from six to 20 and observed heterozygosity ranging from 0.410 to 0.940. Although all loci were in Hardy-Weinberg equilibrium, locus Mst95 showed significant homozygote excess in both surveyed populations, possibly a consequence of the presence of null alleles at this locus. These loci will be used to determine the extent to which spot-throat populations are isolated in order to help set conservation priorities for this ancient African lineage. |
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| Keywords: | Aves Eastern Arc Mountains montane forest Tanzania tetranucleotides |
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