PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the Mediterranean shore crab Carcinus aestuarii (Decapoda, Portunidae) |
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Authors: | Marino Ilaria A M Barbisan Federica Gennari Micol Bisol Paolo Maria Zane Lorenzo |
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Institution: | Department of Biology, University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy. |
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Abstract: | We characterized nine polymorphic microsatellites in the Mediterranean shore crab Carcinus aestuarii (Decapoda: Portunidae). Microsatellites were isolated from a partial genomic library enriched for multiple motifs. All loci were polymorphic, with number of alleles ranging from two to 16 and a mean observed heterozygosity of 0.75. Seven loci were in Hardy-Weinberg equilibrium, and two showed weak heterozygote deficiency. No linkage disequilibrium was found between loci. In addition, we tested Hardy-Weinberg and linkage equilibrium of three Carcinus maenas loci, already reported to cross-amplify in C. aestuarii. These molecular markers will be potentially useful to investigate genetic structure of this species. |
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Keywords: | Carcinus aestuarii Decapoda FIASCO protocol Mediterranean Sea microsatellite |
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