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Isolation and characterization of microsatellite loci in the Guanacaste tree, Enterolobium cyclocarpum
Authors:Peters Maureen B  Hagen Cris  Trapnell Dorset W  Hamrick J L  Rocha Oscar  Smouse Peter E  Glenn Travis C
Affiliation:Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA, Institute of Ecology, University of Georgia, Athens, GA 30602, USA, Department of Plant Biology, University of Georgia, Athens, GA 30602, USA, Department of Biological Sciences, Kent State University, Kent, OH 44242, USA, Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ 08901, USA.
Abstract:We isolated nine microsatellite loci from the Guanacaste tree (Enterolobium cyclocarpum) and optimized them for future research on breeding populations of this species. Loci were screened across 53 individuals from one population and were shown to be variable with the number of alleles per locus ranging from five to 15. Polymorphic information content ranged from 0.420 to 0.900 and observed heterozygosity ranged from 0.547 to 0.906.
Keywords:Enterolobium cyclocarpum  Guanacaste tree  microsatellites  parentage analysis  PCR  pollen dispersal  population structure
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